Letheen agar modified is intended for use in the isolation of microorganisms from cosmetics.
The casein peptone, meat peptone, beef extract and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Glucose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. The sodium bisulfite, lecithin and polysorbate 80 inactivates quaternary ammonium compounds. Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.
Suspend 54.1g of the medium in 1L of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.