HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
- High binding capacity – binds up to 40mg his-tag protein/mL resin.
- Excellent flow properties – great performance even at high flow rates.
- Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins.
- HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.
His-tag protein purification from unclarified lysates
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without pre-centrifugation and filtration.
If you plan to load unclarified samples, it is important to ensure that lysis is as complete as possible. We recommend adding lysozyme and DNase I, followed by mechanical lysis (e.g. by sonication) for a longer duration than typically performed.