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17528601 Display Image

HisTrap FF Crude 5 x 5 ml

Code: 17528601 D2-84

HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.

  • High binding capacity – binds up to 40mg his-tag pr...

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HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.

  • High binding capacity – binds up to 40mg his-tag protein/mL resin.
  • Excellent flow properties – great performance even at high flow rates.
  • Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins.
  • HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.

His-tag protein purification from unclarified lysates

HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without pre-centrifugation and filtration.

If you plan to load unclarified samples, it is important to ensure that lysis is as complete as possible. We recommend adding lysozyme and DNase I, followed by mechanical lysis (e.g. by sonication) for a longer duration than typically performed.

HisTrap FF Crude 5 x 1mL
Bed dimensions, mm7 x 25
Bed height, mm25
Bed volume, mL1
Column i.d.7
Dynamic capacity2
Flow rate, mL/min<4
ApplicationIntended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
Binding Capacity/ColumnApprox. 40mg histidine-tagged protein/mL medium
Order InformationUse 20-40mM imidazole in sample and binding buffers for highest purity
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)
Storage4 to 30°C, 20% Ethanol
Chromatography techniqueHistidine-tagged protein purification

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