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Protein A Sepharose CL-4B 1.5 g

Code: 17078001 D2-84

Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.

  • High binding capacity of ...

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Protein A Sepharose CL-4B is a trusted, well-documented affinity resin for immunoprecipitation procedures. It can also be used for antibody purification.

  • High binding capacity of 20mg human IgG/mL.
  • High selectivity and low nonspecific adsorption.
  • Well suited for immunoprecipitation procedures.

Use in immunoprecipitation procedures

Protein A Sepharose CL-4B resin offers high specificity and capacity, making it dependable for precise, reproducible immunoprecipitation procedures – whether the end goal is determining protein quality, analyzing protein interactions, or assessing post-translational modifications.

Protein A Sepharose CL-4B
Particle Size, um45 - 165
Flow velocity150cm/h at 25°C, HR 16/10 columns, 5cm bed height
LigandNative Protein A
Particle size, d50V~90µm
MatrixCross-linked agarose, 4%
Ligand Coupling MethodCyanogen bromide activation
Binding Capacity/ml Chromatography Medium~20mg human lgG/mL resin
Ligand density3mg Protein A/mL medium
pH stability, operational3 - 9
pH stability, CIP2 - 10
Storage2 to 8°C, 20% Ethanol
Chemical stabilityStable in commonly used aqueous buffers, 6M guanidine-HCl, 8M urea
Sanitization Conditions2% Hibitane/20% Ethanol or with 70% Ethanol
Chromatography techniqueAntibody affinity chromatography

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17096302
EACH
€14,775.80
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17096303
EACH
€2,044.90
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