Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.
For DNA purification from small molecules by gel filtration in spin columns with an exclusion limit of 25bp ds DNA
Tested to ensure reproducibly high recovery of DNA
Suitable for researchers who prefer to prepare their own columns for nucleic acid purification
Sephadex G-50 DNA Grade is used in AutoSeq G-50 columns, ProbeQuant G-50 Micro Columns, MicroSpin G-50 Columns, and NICK Columns
Sephadex G-100 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Spin columns packed with Sephadex G-100 DNA Grade allow for high-recovery nucleic acid purification via gel filtration. Gel filtration resins do not exhibit a fixed exclusion limit when used in a spin-column format. In spin-column chromatography, the observed exclusion properties that allow the product to pass through the gel while the smaller impurities are retained depend on experimental factors, such as the resin used, sample volume, product size, and the g-forces used in the purification process.
Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.