17528001 Display Image
17528001 Display Image

nProtein A Sepharose 4 Fast Flow 200 ml

Code: 17528001 D2-84

nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.

  • For the purification of monoclonal and polyclonal antibodies...

    read more

    ADD
    €703.30 EACH
    List Price

nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.

  • For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
  • Used in routine commercial polyclonal and monoclonal antibody purification and production.
  • Free from animal-derived components.
  • Well suited to immunoprecipitation procedures.
  • Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Use in antibody purification

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well-established Sepharose 4 Fast Flow base matrix. It is used for commercial production but also for research purposes.The high specificity of the native protein for the Fc region of IgG gives a high purification factor.

As a member of the BioProcess media (resins) range, nProtein A Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

ParameternProtein A Sepharose 4 Fast Flow
BioProcess resinYes
Particle size, d50V~90µm
Matrixcross-linked agarose, 4%, spherical
Ligand Coupling MethodCyanogen bromide activation
Binding Capacity/mL Chromatography Medium>35mg human IgG/mL
Ligand density6mg Protein A/mL medium
Total binding capacity≥30mg hIgG/mL chromatography resin
pH stability, operational3 - 9
pH stability, CIP2 - 10
Pressure/flow characteristics150-250cm/h at <0.1MPa in a XK 50/60 column with 5cm diameter and 25cm bed height (at 20?C using buffers with the same viscosity as water)
Storage2 to 8°C, 20% Ethanol
Chemical stabilityStable in aqueous buffers commonly used in Protein A chromatography, 6M guanidine-HCl, 70% ethanol, 3M NaSCN, 0.1M glycine (pH 3.0), 2% benzyl alcohol or 20 % ethanol
Certificate of AnalysisYes
Chromatography techniqueAntibody affinity chromatography

Code
Description
Unit Size
List Price
Qty
17528002
200ML
€4,973.80
ADD
17528004
EACH
€2,450.50
ADD