Sepharose 4B is a well-proven agarose size exclusion chromatography base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
- 4% Agarose size exclusion chromatography resin.
- Proven base matrix for coupling affinity ligands
Sepharose is a spherical agarose-based size exclusion chromatography matrix. Sepharose is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively, Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight
Sepharose CL resins are cross-linked derivatives of Sepharose 2B, Sepharose 4B and Sepharose 6B. The cross-linked form of Sepharose is chemically and physically more resistant than Sepharose itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose gels are resistant to organic solvents and are thus the choice for separations in organic solvents