HiTrap Protein A HP are prepacked columns for routine preparative purification of monoclonal and polyclonal antibodies such as human IgG.
- First choice Protein A resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
- Small bead size (34 µm) ensures narrow elution peaks containing concentrated material
Protein A column for IgG purification
HiTrap Protein A HP is packed with Protein A Sepharose High Performance resin. Use this column for routine immunoaffinity purifications of most IgGs from ascites, serum, and cell culture supernatant.
These columns deliver high-performance, high-resolution purifications with a binding capacity of 20mg IgG/mL resin. Columns are stable over a wide pH range – operational pH 3 to 9 for use; pH 2 to 10 for cleaning.