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17040501 Display Image

HiTrap Protein G HP 1 x 5 ml

Code: 17040501 D2-84

HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.

  • First choice Protei...

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HiTrap Protein G HP are prepacked columns for general-purpose preparative purification of monoclonal and polyclonal antibodies from most species including rat.

  • First choice Protein G resin for routine purification of antibodies: ensures minimized sample dilution and high resolution.
  • The small bead size (34µm) ensures narrow elution peaks containing concentrated material.
  • High protein recovery due to high binding capacity of 25mg IgG/mL resin.
  • Broader selectivity compared with protein A ligand.
  • Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as ÄKTA system.

Protein G column for general-purpose antibody purification

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance resin. This protein G affinity resin is a good first choice for general-purpose capture of antibodies at laboratory scale, because it binds to a broader range of IgG from eukaryotic species and also binds to more subclasses of IgG than protein A-based resins do.

Use these columns for IgG purification from cow, sheep, horse, rat, mouse, and human sources, including ascites, serum, and cell culture supernatants.

HiTrap Protein G HP columns deliver high-performance, high-resolution purifications with a binding capacity of 25mg IgG/mL resin and fast kinetics. Columns are stable over a wide pH range – operational pH 3 to 9, and pH 2 to 10 for cleaning.

HiTrap Protein G HP 5 x 1mLHiTrap Protein G HP 2 x 1mLHiTrap Protein G HP 1 x 5mLHiTrap Protein G HP 5 x 5mL
Bed Dims, mm7 x 257 x 2516 x 2516 x 25
Bed Height, mm25252525
Bed Volume1155
Binding Capacity/Column25mg human IgG/column25mg human IgG/column125mg human IgG/column125mg human IgG/column
Column i.d.771616
Flow Rate, mL/min<4<4>20>20
ApplicationDesigned for use with a syringe, peristaltic pump, or chromatography system
Pressure max. (over the packed bed during operation)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)5 bar [0.5MPa] (70psi)
Storage2 to 8°C, 20% ethanol2 to 8°C, 20% ethanol2 to 8°C, 20% ethanol2 to 8°C, 20% ethanol
Chromatography TechniqueAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatographyAntibody affinity chromatography

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17040401
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€977.60
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17040403
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€384.80
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17040503
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€3,311.10
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