Lentil Lectin Sepharose 4B resin is used for glycoprotein purification and purification of other carbohydrate-containing molecules.
- Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related sugars.
- Compared to Con A, lentil lectin distinguishes less sharply between glucosyl and mannosyl residues.
- Retains binding in 1% deoxycholate and is valuable for purifying detergent-solubilized membrane proteins.
Capture of proteins with affinity for lentil lectin
Lentil lectin is a metalloprotein that binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a group-specific adsorbent that is well-suited to purifying membrane proteins and viral glycoproteins.