Chelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity.
- Based on well-established Sepharose Fast Flow resins.
- High chemical stability enables proven CIP and sanitization protocols.
- Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
Chelating resin for flexible and reliable IMAC purification
Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic, which comes immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms. The highly crosslinked agarose resin enables high flow rates and high productivity.
This immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions (uncharged), allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.