17061805 Display Image

Protein G Sepharose 4 Fast Flow 200 ml

Code: 17061805 L1-23

Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.

  • High binding capacity: approximately 20mg human IgG per mL resin
  • Broad-spect...

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    €5,021.90 EACH
    List Price

Protein G Sepharose Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow resin.

  • High binding capacity: approximately 20mg human IgG per mL resin
  • Broad-spectrum: binds to a broad range of IgG species and subclasses
  • Complements Protein A resins: unlike protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses
  • No specific albumin binding: recombinant protein G has been genetically engineered to remove the albumin binding region
  • Minimal leaching: optimized coupling method minimizes leaching of recombinant protein G and improves binding capacity for IgG
  • Wide range of formats: including loose resin packs from 5mL to 5L, for manual or automated antibody purification and immunoprecipitation procedures

IgG purification

Protein G Sepharose 4 Fast Flow is a good first choice for general-purpose capture of antibodies at laboratory scale. Compared to protein A-based resins, it binds a broader range of immunoglobulins from eukaryotic species and more classes of IgG.

Key applications include:

  • isolation and purification of IgG from serum, ascites, and tissue culture media
  • monoclonal antibody purification
  • isolation of antibody-ligand immune complexes
  • immunoprecipitation protocols

The recombinant protein G ligand binds strongly to the Fc-region from a variety of mammalian species. In addition, many antibodies also interact via the Fab region with a low affinity site on protein G. The ligand is immobilized via a multipoint coupling to the Sepharose 4 Fast Flow agarose matrix to minimize ligand leakage.

Protein G Sepharose 4 Fast Flow resin is part of Cytiva's BioProcess media (resins) range, which ensures security of supply and comprehensive technical and regulatory support.

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17061801
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List Price: €826.80
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17061802
Unit:EACH
List Price: €3,138.20
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