17094801 Display Image
17094801 Display Image

Blue Sepharose 6 Fast Flow 50 ml

Code: 17094801 D2-84

Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This resin is well established as adsorbent for albumin and interferon at both laboratory and process scale

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Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This resin is well established as adsorbent for albumin and interferon at both laboratory and process scale

  • Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity

Blue Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography resins for capture and intermediate purification. Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Blue Sepharose 6 Fast Flow
Particle Size, µm45 - 165
Certificate of AnalysisYes
BioProcess resinYes
LigandCibacron Blue
Particle size, d50V~90µm
Matrix6% cross-linked agarose
Ligand Coupling MethodTriazine
Ligand density~7µmol Cibacron Blue/mL medium
pH stability, operational4 - 12
pH stability, CIP3 - 13
Pressure/Flow Specification250-400cm/h, 100 kPa, XK 50/60 column, bed height 25cm
Storage2 to 8°C, 20% Ethanol, 0.1M Potassium Phosphate buffer, pH 8.0
Chemical stabilityStable in commonly used aqueous buffers - 70% ethanol, 6M guanidine hydrochloric, 8M urea
Chromatography techniqueGroup specific affinity