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IgG Sepharose 6 Fast Flow 10 ml

Code: 17096901 D2-84

lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins.

  • Designed for rapid, single-step ...

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lgG Sepharose 6 Fast Flow uses the rigid Sepharose 6 Fast Flow matrix, covalently coupled with human IgG, to purify protein A-containing proteins.

  • Designed for rapid, single-step purification of protein A and protein A fusion conjugates.
  • Binds at least 2 mg protein A/mL resin.
  • Suitable for tandem affinity purification (TAP) of protein complexes.

IgG Sepharose 6 Fast Flow
Flow velocity<300cm/h, using XK 50/30 column with 15cm bed height
LigandHuman IgG
Particle size, d50V~90µm
MatrixHighly cross-linked 6% agarose
Binding Capacity/ml Chromatography Medium~3mg Protein A
ApplicationRecombinant tagged proteins containing a protein A tag
pH stability, operational3 - 10
pH stability, CIP3 - 10
Pressure/Flow SpecificationBase matrix: 250-400cm/h, 100kPa, XK 50/60 column, bed height 25cm
Storage2 to 8°C, 20% Ethanol, 50mM K2HPO4, 1mM MgCl2 pH 7.2 in 20% Ethanol
Chemical stabilityStable to commonly used aqueous buffers