17513102 Display Image
17513102 Display Image

GSTrap FF 5 x 5mL

Code: 17513102 D2-84

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

  • Fast and simple one-step purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose 4 Fast Flow provides good flow properties.

GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.

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Description
Unit Size
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17513001
Unit:EACH
List Price: €375.70
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17513101
Unit:EACH
List Price: €358.80
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17513002
Unit:EACH
List Price: €184.60
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