Blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
- High-quality genomic DNA extraction from blood with a greater proportion of higher molecular weight DNA and less shearing
- Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
- Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
- Verified in several downstream applications including reverse transcription polymerase chain reaction (RT-PCR), endpoint PCR, multiplex PCR, and restriction enzyme digests
Genomic DNA is found in the chromosomes and it is much longer and higher in molecular weight than plasmid DNA. Genomic DNA is the code for the genome, and this is what is needed to do genome sequencing.
For sequencing it is desirable that the genomic DNA extraction technique used allows the collection of intact genomic DNA in the longest fragments possible. A kit format can help standardize the procedures used to prepare tissue and cell genomic DNA samples for analysis.
The purification process involves minimal shearing resulting in the production of 5 to 10µg of good quality, intact genomic DNA from a 200µL sample. Input sample volumes can vary from 50µL to 1mL.
The method uses a simple genomic DNA purification protocol. This protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. This results in high molecular weight DNA that can be used downstream (e.g., in bone marrow genomic DNA for next generation sequencing).
The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.
The genomic DNA kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.