356001

Growth factors are proteins that bind to receptors found on the surface of non-hematopoietic cells. Each family of growth factors affect specific cell types, e.g., nerve growth factors (NGF) affect nerve cell types, and epidermal growth factors (EGF) affect epithelial cell types.

Corning offers a range of growth factors that are derived from different species, both natural and recombinant. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions. Stable for at least three months at 2° to 8°C (lyophilized) or three months at -20°C (solubilized), do not store in frost-free freezer, avoid multiple freeze/thaws.

Mouse Natural

Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O. Sourced from male mouse submaxillary glands. Purity: >95% by SDS-PAGE and >80% by pH 9.9 PAGE, tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium, filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma. Molecular weight: 6.1 kD. Recommended concentration: 5 to 100 ng/ mL medium depending on cell type.

Human Recombinant

Lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer. Purity: >98% by SDS-PAGE and reverse phase HPLC, shown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium, filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma. Molecular weight: 6.2 kD. Recommended concentration: 0.1-1.0 ng/ mL medium depending on cell type.

Formulation (mouse natural): lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O
Formulation (human recombinant): lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer
Preparation and storage:
Purity: >98% by SDS-PAGE and reverse phase HPLC
Shown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Source (mouse natural): male mouse submaxillary glands
Source (human recombinant): recombinant, E. coli
Recommended concentration: 0.1-1.0 ng/ mL medium depending on cell type