Human plasma contains a wide variety of proteins that hold potential as biomarkers for proteomics-based diagnostic and prognostic assays. These proteins are subject to proteolytic degradation and modification during and after blood collection. The BD™ P100 blood collection tubes minimize preanalytical variability in plasma protein analysis. The tubes are spray coated with K2EDTA anticoagulant and proprietary protease inhibitor cocktail, specifically formulated for stabilization of human plasma proteins at the point of collection. Inclusion of a novel mechanical separator within the 8.5mL BD™ P100 tubes provides better separation of cellular components from plasma than gel-based separators.