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2-Mercaptoethanol, BioUltra, for molecular biology, >=99.0% (GC)

Code: 63689-100ML-F D2-231

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a conc...


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Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

2-Mercaptoethanol has been used in the protein extraction buffer prepared for leaf samples. as a supplement in cell culture media. in the procedure of RNA extraction.

Other Notes

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

λ0.5 M in H2O
assay≥99.0% (GC)
bp157 °C (lit.)
cation tracesSr: ≤0.1 mg/kg, Cr: ≤0.02 mg/kg, Ca: ≤0.5 mg/kg, Ni: ≤0.05 mg/kg, Cd: ≤0.05 mg/kg, Bi: ≤0.1 mg/kg, K: ≤0.5 mg/kg, Al: ≤0.5 mg/kg, Na: ≤1 mg/kg, Ba: ≤0.1 mg/kg, Mg: ≤0.1 mg/kg, Zn: ≤0.1 mg/kg, Mo: ≤0.1 mg/kg, Li: ≤0.1 mg/kg, Mn: ≤0.02 mg/kg, Co: ≤0.02 mg/kg, Cu: ≤0.02 mg/kg, Pb: ≤0.1 mg/kg, Fe: ≤0.1 mg/kg
concentration14.3 M (pure liquid)
density1.114 g/mL at 25 °C (lit.)
expl. lim.18 %
formliquid
gradefor molecular biology
impuritiesphosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI keyDGVVWUTYPXICAM-UHFFFAOYSA-N
InChI1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
pH4.5-6 (20 °C, 500 g/L)
product lineBioUltra
Quality Level200
refractive indexn20/D 1.501, n20/D 1.500 (lit.)
SMILES stringOCCS
solubilityH2O: 0.5 M at 20 °C, clear, colorless
storage temp.2-8°C
technique(s)protein extraction: suitable, RNA extraction: suitable
UV absorptionλ: 260 nm Amax: 1.5, λ: 280 nm Amax: 0.3
vapor density2.69 (vs air)
vapor pressure1 mmHg ( 20 °C)
Code
Description
Unit Size
List Price
Qty
63689-25ML-F
2-Mercaptoethanol, BioUltra, for molecular biology, >=99.0% (GC)

Unit:25ML
List Price: €35.70
Source:List Price
ADD
Cas Number60-24-2
Hazard Class6.1
Un Number2966
Pack GroupII
This product has met the following criteria: