Application

Gamborg’;s B-5 medium has been used for the culture of Annona squamosa callus.

Gamborg′s B-5 Basal Salt Mixture has been used: as a constituent during the preparation of TEX buffer as a media to maintain potato suspension and callus cultures as a media in the primary-node transformation method

Formula variant

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).Media Formulation

General description

Gamborg’; B-5 medium described by Gamborg, et al. may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’;s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Quantity

Formulated to contain 3.1 grams of powder per liter of medium.