Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Reducing agentβ-mercaptoethanol or 2-mercaptoethanol has been used:as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)to lyse NuLi-1 (Normal Lung, University of Iowa-1) cellsto trypsinize mouse embryonic stem cells

Packaging

25, 100, 250, 500 mL in glass bottle