Application

The antibody may be used in:immunoblotting immunoprecipitationimmunohistochemistry immunocytochemistry flow cytometryimmunofluorescence

Biochem/physiol Actions

Myosin implicated in cell motility. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. In fact, the thick filament consists mainly of myosin molecules. The ATPase of myosin is activated by actin. This activation is the immediate source of the free energy that drives muscle contraction. It binds to the polymerized form of actin, the major constituent of the thin filament. Myosin is generally assumed to be a very specific and reliable smooth muscle marker, provided that the antibody used is specific for smooth muscle myosin and does not cross-react with skeletal, cardiac or non-muscle myosins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Myosin (Smooth) (mouse IgG1 isotype) is derived from the hSM-V hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human uterus smooth muscle extract. Myosin (~500 kDa) is a cytoskeletal protein containing two identical heavy chains (~200 kDa each) and four light chains (15-26 kDa). Myosin molecules consist of two major regions: tail (rod) and heads.

Immunogen

human uterus smooth muscle extract.

Specificity

Mouse monoclonal clone hSM-V anti-Myosin (Smooth) antibody reacts in immunoblotting assays with myosin heavy chain polypeptides of 204 and 200 kDa (SM-1 and SM-2). It does not cross-react with skeletal, cardiac or non-muscle myosin. The antibody stains vascular and visceral smooth muscle cells, as well as cells that have smooth muscle-like characteristics (myofibroblasts and myoepithelial cells). It does not recognize epithelial, endothelial or connective tissue fibroblast cells. The product reacts with smooth muscle tissue from human, dog, rabbit, pig, guinea pig, and chicken.