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E and O Labs Letheen Agar Modified

Code: MED1656 D2-65

Letheen agar modified is intended for use in the isolation of microorganisms from cosmetics.

The casein peptone, meat peptone, beef extract and yeast extract act as carbon, nitrogen and vitam...

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Letheen agar modified is intended for use in the isolation of microorganisms from cosmetics.

The casein peptone, meat peptone, beef extract and yeast extract act as carbon, nitrogen and vitamin sources in this medium. Glucose is a fermentable carbohydrate. Sodium chloride maintains the osmotic balance of the medium. The sodium bisulfite, lecithin and polysorbate 80 inactivates quaternary ammonium compounds. Polysorbate 80 neutralises phenols, formalin, hexachlorophene, and in combination with the lecithin, ethanol.

Preparation

Suspend 54.1g of the medium in 1L of deionised / purified water. Allow the medium to soak whilst mixing for 10 minutes. Sterilise at 121°C for 15 minutes. Cool to 45-50°C and aseptically dispense into appropriate sterile containers.

MED1656
Shelf life, days1095
Storage temp, °C10 - 30
Dehydrated medium appearancePowder
Prepared medium appearanceFirm gel
Dehydrated medium colourStraw
Prepared medium colourStraw
Dehydrated pH7.2 ±0.2
Prepared pH7.2 ±0.2
Recommended incubationAerobically at 37°C ±1 for 18-24hr
Formula
Casein peptone, g/L10
Meat peptone, g/L10
Polysorbate 80, g/L7
Sodium chloride, g/L5
Beef extract, g/L3
Yeast extract, g/L2
Glucose, g/L1
Lecithin, g/L1
Sodium bisulfite, g/L0.1
Agar, g/L15
Total, g/L54.1

Pack500g