The UreaGel System consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the UreaGel System the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 acrylamide: bisacrylamide).
Litre bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution. UreaGel Diluent is supplied in 450mL and 1 litre bottles containing 7.5M urea in deionized water. UreaGel Buffer is supplied in 100mL and 200mL bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH 8.3 (10X TBE) and 7.5M urea.
Storage: UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature (20°C).
Keep in a tightly closed container, stored in a cooled, dry, ventilated area. Protect from physical damage. Isolate from incompatible materials. Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.
- Casts 19:1 denaturing gels up to 20% monomer
- Certified RNase and DNase free
- Consistently crystal clear gels
- Twelve month shelf life at room temperature
- Requires addition of TEMED and ammonium persulfate prior to casting
2.2L Sequencing System to Cast Denaturing Gels contain: 1L concentrate, 1L Diluent and 200mL buffer
1L Sequencing System to Cast Denaturing Gels contain: 450mL concentrate, 450mL Diluent and 100mL buffer