The SensiMix™ SYBR® Hi-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require high concentrations of the passive reference dye ROX for normalization of well-to-well differences.
A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® Hi-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.
The SensiMix SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
- Sensitive - reproducible detection of low-copy number templates
- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
- Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
- Applications: Gene expression analysis, DNA / cDNA target detection, miRNA profiling / quantification, and copy number variation (CNV) analysis
- SensiMix SYBR® Hi-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and ROX for optional use
- QT60505: 500 x 50 µL Reactions: 10 x 1.25 mL
- QT60520: 2000 x 50 µL Reactions: 40 x 1.25 mL
- Storage: -20°C upon receipt for optimum stability, Repeated freeze/thaw cycles should be avoided, avoid exposure of the SYBR® Green I to light.
- Stability: When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
This product has met the following criteria: